3D CULTURE PLATFORM OF HUMAN IPSCS-DERIVED NOCICEPTORS FOR PERIPHERAL NERVE MODELLING AND TISSUE INNERVATION
The ability to detect external noxious stimuli and signals from internal organs is essential to maintain physical integrity and homeostasis. This process is mediated by nociceptors, which recognize stimuli through specialized receptors. The perception of nociceptive pain depends on the continuity of the sensory fibers of the peripheral nerve, which is why functional humanized in vitro nerve models are coveted as an alternative to animal models due to their ease of access, lower cost, clinical relevance and the no need for recurring animal sacrifices. To this end, a sensory nerve model was developed using induced pluripotent stem cell (iPSC) derived nociceptors that are electrically active and exhibit a functional response to noxious stimuli.
Afonso Malheiro 1, Abhishek Harichandan 2, Joyce Bernardi 3, Adrián Seijas-Gamardo 1, Gonda F Konings 4, Paul G A Volders 3, Andrea Romano 4, Carlos Mota 5, Paul Wieringa 6, Lorenzo Moroni 7